Single Molecule Reaction Kinetics within Femtoliter Volume Containers
نویسندگان
چکیده
منابع مشابه
Single molecule kinetics of horseradish peroxidase exposed in large arrays of femtoliter-sized fused silica chambers.
Large arrays of femtoliter-sized chambers were etched into the surface of fused silica slides to enclose and observe hundreds of single horseradish peroxidase (HRP) molecules in parallel. Individual molecules of HRP oxidize the fluorogenic substrate Amplex Red to fluorescent resorufin in separate chambers, which was monitored by fluorescence microscopy. Photooxidation of Amplex Red and photoble...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2009
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2008.12.3176